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22 July 2020 Molecular Diagnosis and Prevalence of Trypanosoma vivax (Trypanosomatida: Trypanosomatidae) in Buffaloes and Ectoparasites in the Brazilian Amazon Region
Gustavo Henrique Souza Dyonisio, Helder Ribeiro Batista, Ryan Emiliano da Silva, Roberta Carvalho de Freitas e Azevedo, Jaciara de Oliveira Jorge Costa, Ingridi Braz de Oliveira Manhães, Renata Tonhosolo, Solange Maria Gennari, Antonio Humberto Hamad Minervino, Arlei Marcili
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Abstract

Trypanosoma vivax Ziemann is a parasite that affects both wild and domestic ungulates and is transmitted mechanically via tabanids and other blood-sucking insects in the Americas. A total of 621 blood samples from water buffaloes (Bubalus bubalis (Linnaeus) (Artiodactyla: Bovidae), and 184 ectoparasite samples (Amblyomma cajennense (Fabricius) sensu stricto and Rhipicephalus (Boophilus) microplus (Canestrini) (Acari: Ixodidae), and Haematopinus tuberculatus (Burmeister) (Phthiraptera: Haematopinidae)) were obtained from 60 farms in the State of Pará, Brazilian Amazon. Twelve buffalo blood samples (1.89%) and 11 ectoparasites (6%) were positive for T. vivax based on the cathepsin L-like gene. All sequences were 99% similar to T. vivax from northeastern Brazil (EU753788) in amplified PCR assays on each of the hosts tested.

© The Author(s) 2020. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Gustavo Henrique Souza Dyonisio, Helder Ribeiro Batista, Ryan Emiliano da Silva, Roberta Carvalho de Freitas e Azevedo, Jaciara de Oliveira Jorge Costa, Ingridi Braz de Oliveira Manhães, Renata Tonhosolo, Solange Maria Gennari, Antonio Humberto Hamad Minervino, and Arlei Marcili "Molecular Diagnosis and Prevalence of Trypanosoma vivax (Trypanosomatida: Trypanosomatidae) in Buffaloes and Ectoparasites in the Brazilian Amazon Region," Journal of Medical Entomology 58(1), 403-407, (22 July 2020). https://doi.org/10.1093/jme/tjaa145
Received: 16 April 2020; Accepted: 19 June 2020; Published: 22 July 2020
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KEYWORDS
Amazonia
lice
molecular diagnosis
tick
trypanosomiasis
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