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18 January 2021 From the Field to the Laboratory: Quantifying Outdoor Mosquito Landing Rate to Better Evaluate Topical Repellents
Mara Moreno-Gómez, Rubén Bueno-Marí, Andrea Drago, Miguel A. Miranda
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Abstract

Vector-borne diseases are a worldwide threat to human health. Often, no vaccines or treatments exist. Thus, personal protection products play an essential role in limiting transmission. The World Health Organization (WHO) arm-in-cage (AIC) test is the most common method for evaluating the efficacy of topical repellents, but it remains unclear whether AIC testing conditions recreate the mosquito landing rates in the field. This study aimed to estimate the landing rate outdoors, in an area of Europe highly infested with the Asian tiger mosquito (Aedes albopictus (Skuse, 1894, Diptera: Culididae)), and to determine how to replicate this rate in the laboratory. To assess the landing rate in the field, 16 individuals were exposed to mosquitoes in a highly infested region of Italy. These field results were then compared to results obtained in the laboratory: 1) in a 30 m3 room where nine volunteers were exposed to different mosquito abundances (ranges: 15–20, 25–30, and 45–50) and 2) in a 0.064 m3 AIC test cage where 10 individuals exposed their arms to 200 mosquitoes (as per WHO requirements). The highest mosquito landing rate in the field was 26.8 landings/min. In the room test, a similar landing rate was achieved using 15–20 mosquitoes (density: 0.50–0.66 mosquitoes/m3) and an exposure time of 3 min. In the AIC test using 200 mosquitoes (density: 3,125 mosquitoes/m3), the landing rate was 229 ± 48 landings/ min. This study provides useful reference values that can be employed to design new evaluation standards for topical repellents that better simulate field conditions.

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© The Author(s) 2021. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Mara Moreno-Gómez, Rubén Bueno-Marí, Andrea Drago, and Miguel A. Miranda "From the Field to the Laboratory: Quantifying Outdoor Mosquito Landing Rate to Better Evaluate Topical Repellents," Journal of Medical Entomology 58(3), 1287-1297, (18 January 2021). https://doi.org/10.1093/jme/tjaa298
Received: 25 September 2020; Accepted: 16 December 2020; Published: 18 January 2021
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KEYWORDS
Aedes albopictus
arm in cage
field testing
laboratory testing
LRC
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