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9 February 2022 Characterized Gene Repertoires and Functional Gene Reference for Forensic Entomology: Genomic and Developmental Transcriptomic Analysis of Aldrichina grahami (Diptera: Calliphoridae)
Fanming Meng, Han Han, Mo Wang, Yangshuai Jiang, Zhiyun Pi, Yihong Qu, Zhuoying Liu, Jifeng Cai
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Abstract

Many flies of Diptera are common entomological evidence employed in forensic investigation. Exploring the existence of inter- and intra-species genomic differences of forensically relevant insects is of great importance. Aldrichina grahami is a common blow fly species of forensic importance. The present study characterized the gene repertoires of A. grahami, and provides insights into issues related to forensic entomology, such as necrophagous behavior, gene family features, and developmental patterns. Gene families were clustered and classified according to their function in different aspects of the necrophagous lifestyle, generating several gene repertoires. The genes under positive selection pressure and evolutionary changes were screen and identified. Moreover, genes that exhibited potential prediction value in the post mortem interval (PMI) estimation and development of immature stages were subjected to analysis based on the developmental transcriptome. Related insect species were compared at the genomic level to reveal the genes associated with necrophagous behaviors. The expression of selected genes in separated repositories was verified using qPCR. This work was conducted using a high-quality chromosome-level genome assembly of A. grahami and its developmental transcriptome. The findings will facilitate future research on A. grahami and the other forensically important species.

© The Author(s) 2022. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Fanming Meng, Han Han, Mo Wang, Yangshuai Jiang, Zhiyun Pi, Yihong Qu, Zhuoying Liu, and Jifeng Cai "Characterized Gene Repertoires and Functional Gene Reference for Forensic Entomology: Genomic and Developmental Transcriptomic Analysis of Aldrichina grahami (Diptera: Calliphoridae)," Journal of Medical Entomology 59(3), 810-819, (9 February 2022). https://doi.org/10.1093/jme/tjac004
Received: 17 March 2021; Accepted: 6 January 2022; Published: 9 February 2022
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KEYWORDS
Aldrichina grahami
blow fly
forensic entomology
genomic
postmortem interval
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