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19 April 2023 Metagenomic profile of the bacterial communities associated with Ixodes granulatus (Acari: Ixodidae): a potential vector of tick-borne diseases
Ernieenor Faraliana Che Lah, Mariana Ahamad, Apanaskevich Dmitry, Badrul Munir Md-Zain, Salmah Yaakop
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Abstract

Ixodes granulatus Supino, 1897 (Acari: Ixodida) is one of Malaysia's most common hard ticks and is a potential vector for tick-borne diseases (TBDs). Despite its great public health importance, research on I. granulatus microbial communities remains largely unexplored. Therefore, this study aimed to investigate the bacterial communities of on-host I. granulatus collected from three different recreational areas on the East Coast of Peninsular Malaysia using high throughput Next Generation Sequencing (NGS). A total of 9 females on-host I. granulatus were subjected to metabarcoding analysis targetingV3–V4 regions of 16S ribosomal RNA (rRNA) using the Illumina MiSeq platform.This study identified 15 bacterial phyla corresponding to 19 classes, 54 orders, and 90 families from 435 amplicon sequence variants (ASVs), revealing a diverse bacterial community profile. Together with 130 genera assigned, local I. granulatus harbored 4 genera of pathogens, i.e., Rickettsia da Rocha Lima, 1916 (Rickettsiales: Rickettsiaceae) (58.6%), Borrelia Swellengrebel 1907 (Spirochaetales: Borreliaceae) (31.6%), BorreliellaAdeolu and Gupta 2015 (Spirochaetales: Borreliaceae) (0.6%), and Ehrlichia Cowdria Moshkovski 1947 (Rickettsiales: Ehrlichiaceae) (39.9%). Some endosymbiont bacteria, such as Coxiella (Philip, 1943) (Legionellales: Coxiellaceae), Wolbachia Hertig 1936 (Rickettsiales: Ehrlichiaceae), and Rickettsiella Philip, 1956 (Legionellales: Coxiellaceae), were also detected at very low abundance. Interestingly, this study reported the co-infection of Borrelia and Ehrlichia for the first time, instilling potential health concerns in the context of co-transmission to humans, especially in areas with a high population of I. granulatus.This study successfully characterized the tick microbiome and provided the first baseline data of I. granulatus bacterial communities in Malaysia.These results support the need for way-forward research on tick-associated bacteria using NGS, focusing on medically important species toward TBD prevention.

Ernieenor Faraliana Che Lah, Mariana Ahamad, Apanaskevich Dmitry, Badrul Munir Md-Zain, and Salmah Yaakop "Metagenomic profile of the bacterial communities associated with Ixodes granulatus (Acari: Ixodidae): a potential vector of tick-borne diseases," Journal of Medical Entomology 60(4), 753-768, (19 April 2023). https://doi.org/10.1093/jme/tjad044
Received: 7 December 2022; Accepted: 28 March 2023; Published: 19 April 2023
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KEYWORDS
bacterial
Borrelia
Ixodes granulatus
next-generation sequencing
vector
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