Flier (Centrarchus macropterus: Centrarchidae) were collected from a channel habitat, a prairie habitat, and a boundary between the 2 habitat types in March 2009 and examined for parasites. Flier from the prairie site had a significantly lower abundance of Pterocleidus acer and a significantly higher abundance of Hysterothylaceum juveniles. Patterns of infracommunity similarity showed a distinct break between the 2 habitat types, with fish collected from the boundary site clustering with either channel or prairie communities, suggesting that the small home range of flier restricts the exposure of individuals to parasites.