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1 September 2015 Estimating Natal Origins of Migratory Juvenile Golden Eagles Using Stable Hydrogen Isotopes
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The difficulty in determining the geographic origins of migratory birds and identifying their source populations has limited the understanding of the ecology of many North American species (Viverette et al. 1996, Meehan et al. 2001, Hobson et al. 2009). In North America, Golden Eagles (Aquila chrysaetos) are widespread; they breed predominately in the West from northern Alaska to central Mexico, and occupy a wide range of habitats from arctic tundra to deserts (Kochert et al. 2002). Most raptors, including Golden Eagles, typically occur in low breeding densities, have large home ranges, and nest in remote areas, making it difficult to assess populations using traditional methods such as Breeding Bird Surveys and Christmas Bird Counts (Fuller and Mosher 1981, Titus and Fuller 1990, Rosenfield et al. 1991, Meehan et al. 2001). Consequently, annual migration counts along ridges during fall migration are the traditional means of monitoring migratory Golden Eagle populations. Analysis of long-term migration count data suggests a downward trend in observed numbers for this species. This has raised concern about the status of migratory Golden Eagles in western North America (Kochert and Steenhof 2002, Hoffman and Smith 2005, Smith et al. 2008, Tilly 2008). However, interpreting migration count data can be difficult (McCaffery and McIntyre 2005) and requires knowledge of the source populations, which is incomplete for a majority of migration count sites (Hoffman and Smith 2003). By identifying the geographic origins of the avian migrants, it may be possible to relate migration count trends to a particular regional population of interest (Viverette et al. 1996, Meehan et al. 2001).

Recently, there has been a call for more information on Golden Eagles in the West (Pagel et al. 2010). Downward trends in Golden Eagle numbers at migration count sites may be the result of factors on breeding or wintering grounds, or both, across western North America (Kochert and Steenhof 2002, Hoffman and Smith 2005, Smith et al. 2008, Pagel et al. 2010). However, one of the biggest obstacles in accurately interpreting raptor migration counts in relation to population status is determining origins and destinations of migrants, and this poses difficulties when addressing conservation issues and developing management strategies (Kochert and Steenhof 2002, Hoffman and Smith 2005).

Stable hydrogen isotope analysis utilizing the weighed growing season average precipitation (δ2Hp) can help reveal natal origins of hatch-year (hereafter referred to as juvenile) Golden Eagles captured at annual migration count sites or on wintering grounds. In addition, it can be used to estimate the natal origins of eagles found as mortalities (e.g., on wind farms) and determine whether those individuals were from northern migratory or nonmigratory populations. The analysis utilizes predictable continent-wide distributions of deuterium abundance occurring in rainfall. These geographically specific ratios of deuterium are then transferred from precipitation through the food chain to upper trophic level consumers such as birds and mammals (Hobson and Wassenaar 1997, 1999; Hobson 2005; Wassenaar 2008). A number of studies have used stable hydrogen isotope ratios to estimate the breeding latitudes of Neotropical migratory songbirds (e.g., Kelly et al. 2002, Rubenstein et al. 2002, Wassenaar and Hobson 2000a), and more recently an increasing number of raptor species (Meehan et al. 2001, Lott et al. 2003, Meehan et al. 2003, Smith et al. 2003, DeLong et al. 2005, Smith and Dufty 2005, Hobson et al. 2009, Ruyck et al. 2013, Wittenberg et al. 2013). Although relatively new, stable hydrogen isotope analysis has proven to be successful and revolutionized our understanding of migratory ecology for many avian species (Meehan et al. 2001, Kelly et al. 2005, Wunder et al. 2009, VanWilgenburg and Hobson 2011, but see Smith et al. 2009).

We used stable hydrogen isotope analysis to address three questions about Golden Eagle migratory ecology: (1) can patterns in natal origins of juvenile Golden Eagles captured during fall migration be identified? (2) do migrant juvenile Golden Eagles exhibit an association between latitudinal origin and passage date? and (3) are there any sex-related differences concerning latitudinal origins and passage dates?

Study Area

The Rocky Mountain Front (RMF) stretches from Alaska, U.S.A. to Mexico and holds the largest known concentration of migratory Golden Eagles known on earth (Tilly 2008, Sherrington 2009). The interface of the Rocky Mountains and Great Plains creates a recognizable migration corridor from Alaska to Mexico. In Montana, it is characterized by north-south ridges often leading to distinct geographic bottlenecks where migrating raptors concentrate. We conducted our research at three sites along the RMF in Montana during fall migration: (1) Rogers Pass (47°4.52′N, 112°22.73′W) on the Continental Divide near Lincoln, Montana; (2) Nora Ridge (47°1.14′N, 112°24.28′W) 6.5 km southwest of Rogers Pass and; (3) Grassy Mountain (46°18.74′N, 111°7.13′W) located in the Big Belt Mountains approximately 128 km southeast of Rogers Pass. All three sites are located in the Helena National Forest and are representative of the RMF flyway in Montana.

Methods

We captured juvenile Golden Eagles using bow-nets with Rock Pigeons (Columba livia) as lures (Bloom 1987). We began trapping each year on approximately 15 September and continued daily, through 31 October, 2004–2007. Daily observations and trapping efforts began at approximately 0930 H and finished at approximately 1700 H, weather permitting. We identified juvenile eagles in the hand by plumage (Bloom and Clark 2001). We determined sex via morphometric measurements (Bortolotti 1984, Edwards and Kochert 1986). We validated sex determinations based on morphometric measurements for a subset of our sample via DNA analysis (Zoogen Services, Inco., Davis, California U.S.A.).

We collected 3-cm feather clippings from the crural (upper leg) region of each Golden Eagle captured because of feather abundance in the crural region and ease of sampling. We banded captured individuals with a unique United States Geological Survey (U.S.G.S.) aluminum leg band and fitted them with alphanumerically coded vinyl wing-tags. We sent feather samples to the Colorado Plateau Stable Isotope Laboratory for stable isotope analysis. To limit variability, only the distal end of the non-rachis vane material was analyzed (Wassenaar and Hobson 2006). Using standardized lab protocols, feathers were first cleaned with chloroform-methanol solution and then air-dried to enable exchangeable hydrogen to equilibrate with ambient laboratory moisture, equilibrating for a minimum of 7–10 d prior to analysis (Chamberlain et al. 1997, Wassenaar and Hobson 2000b, 2003). Feathers were then packed in silver capsules and pyrolized using a Themo Finnigan TC/EA elemental analyzer (Thermo Electron Corporation, Boston, Massachusetts, U.S.A.) and sample hydrogen content was analyzed using a Delta Plus XL mass spectrometer (Thermo Electron Corporation, Boston, Massachusetts, U.S.A.) in continuous flow mode at 1400°C. The non-exchangeable hydrogen isotope ratios were calculated via comparative equilibration using keratin standards after Wassenaar and Hobson (2003). Values of δ2Hf were normalized on the VSMOW-SLAP scale using three powdered keratin standards obtained from Environment Canada, Saskatoon, Saskatchewan: bowhead whale baleen (BWB: δ2H  =  −108‰), chicken feather standard (CFS: δ2H  =  147.5‰), and cow hoof standard (CHS: δ2H  =  −187‰; L. Wassenaar pers. comm.). Analytical error of non-exchangeable hydrogen isotope values was ±4%.

Lott and Smith (2006) used over 250 known-origin raptor feathers to create a raptor-specific reference map of δ2Hf for North America. Because there can be differences in δ2Hf detection between labs or even within the same lab from different sample runs, we calibrated our samples using a smaller subset of known-origin samples used by Lott and Smith to make their map (n  =  22). For an anecdotal comparison, we sampled feathers from two Golden Eagles that had been in captivity >10 yr in Montana and fed exclusively local fauna (K. Davis pers. comm.). Both individuals had isotope values of −114‰, which supported established isotope levels for Montana. We measured δ2Hf at our lab (Northern Arizona State Isotope Laboratory) for 22 known-origin feathers that Lott and Smith used to create their reference map. We then used simple linear regression to relate the δ2Hf measured at our lab to the δ2Hf measured at the Lott and Smith lab. We used the regression equation to calibrate our samples from juvenile Golden Eagles. As suggested by Lott and Smith (2006), we incorporated error into the prediction by assessing an upper and lower range for the δ2Hf values of ±8‰.

To map the estimated origin of the captured eagles, we grouped the calibrated δ2Hf values into three classes for ease of display, and reported the percent of birds estimated to originate from each class. We plotted these three classes using data from the Lott and Smith (2006) raptor-specific isoscape reference map, to spatially interpret natal origins of migratory juvenile Golden Eagles. We tested whether juvenile Golden Eagles exhibited migratory timing patterns using simple linear regression with capture date as the independent variable and δ2Hf as the dependent variable. Additionally, we tested for sex differences in δ2Hf using a two sample t-test.

Results and Discussion

We captured and collected feather samples from 50 juvenile Golden Eagles during fall migration. We sampled 12 eagles in 2004 and 13 in 2005 from our Rogers Pass study site, 5 in 2006 and 14 in 2007 from Nora Ridge, and 6 in 2007 from Grassy Mountain. We used a subsample of 25 eagles to verify our sex determinations from morphometrics via DNA tests and 100% of sex assignments were correct. We found that our analysis of the reference feather samples slightly underestimated δ2Hf compared to Lott and Smith (2006) but still exhibited good fit with these data (P < 0.0001, r2  =  0.937; Fig. 1). Consequently, we used the regression equation i0892-1016-49-3-308-e01.gif= 32:92+1:18x to calibrate our results to the raptor-specific map of δ2Hf where x is the measured δ2Hf (‰) and i0892-1016-49-3-308-e02.gif is the calibrated δ2Hf (‰).

Figure 1. 

Relationship between the δ2Hf measured in the known origin samples at our lab (Northern Arizona State Isotope Laboratory) to the δ2Hf reported for the same sample Lott and Smith used to create the raptor specific reference map.

i0892-1016-49-3-308-f01.tif

The mean predicted δ2Hf for all juvenile Golden Eagle feather samples was −145.5‰ (range −103.3‰ to −176.4‰, SD  =  16.4‰; Fig. 2). We incorporated error into the prediction by assessing an upper and lower range for the δ2Hf values of ±8‰ (Fig. 3). Our results indicated 46% (n  =  23) of migratory, juvenile Golden Eagles we sampled had predicted δ2Hf values ≤ −150‰ with possible percentages ranging from 26% to 62% after accounting for error. Additionally, 20% (n  =  10; range 14 to 24%) had predicted δ2Hf values between −150‰ and −140‰. Thus, 66% had predicted natal areas located in the Yukon and western Northwest Territories, Canada, to eastern Alaska (Fig. 4). Based on the isoscape map, it is possible that some individuals may have come from the region of the eastern Northwest Territories and Nunavut provinces, Canada; however, it is unlikely given limited breeding records from that area (Kochert et al. 2002). Furthermore, concurrent satellite tracking studies of adult Golden Eagles captured at our study sites (n  =  18) confirmed summer ranges were all west of central Northwest Territories (R. Domenech unpubl. data). We found no association between time of capture and calibrated δ2Hf (P  =  0.2928) or between sex and calibrated δ2Hf (P  =  0.1349) or sex and time of capture (P  =  0.4404; Fig. 5).

Figure 2. 

Distribution of δ2Hf (‰) in juvenile Golden Eagles captured during migration along the Rocky Mountain Front in Montana (mean  =  –145.5‰, SD = 16.4‰, n = 50). The shades of the bins on the histogram indicate the predicted natal origin regions in Fig. 4.

i0892-1016-49-3-308-f02.tif

Figure 3. 

Predicted δ2Hf values (‰) for the juvenile, migratory Golden Eagles sampled. We incorporated error into the prediction by assessing an upper and lower range for the δ2Hf values of ±8‰.

i0892-1016-49-3-308-f03.tif

Figure 4. 

Raptor-specific deuterium map adapted from Lott and Smith (2006) indicating natal origin regions for juvenile, migratory Golden Eagles captured along the Rocky Mountain Front in Montana.

i0892-1016-49-3-308-f04.tif

Figure 5. 

Relationship between time of arrival and calibrated δ2Hf (‰) and gender and calibrated δ2Hf. Females are represented by a dark circle, males are represented by an open square.

i0892-1016-49-3-308-f05.tif

Previously, researchers utilizing hydrogen stable isotope technology for animal migration studies relied on using a simple model of hydrogen stable isotopes in precipitation (δ2Hp) that did not account for elevation, latitudinal and other sources of variation (Meehan et al. 2003, Lott and Smith 2006). Lott and Smith (2006) developed the raptor-specific deuterium map to allow for more accurate estimation of natal origins of raptors by incorporating both elevationally explicit growing-season precipitation data (Bowen et al. 2005) and an actual known-origin raptor feather base map. We found that plotting calibrated deuterium ratios on the Lott and Smith (2006) base map was useful for estimating natal origins of juvenile Golden Eagles migrating along the RMF in Montana. The results were further supported by several years of satellite tracking data of Golden Eagles using the RMF (R. Domenech unpubl. data).

We acknowledge there are some pitfalls to be considered when applying these techniques which include: the age of the study species, prey types, natal origins in proximity to large bodies of water (e.g., along ocean shorelines) and other variables (Smith et al. 2009, Ruyck et al. 2013, Whittenberg et al. 2013). Smith et al. (2009) report concerns with between-lab reproducibility of δ2Hf. Although there was some variability between the two labs analyzing the same feathers, the values in our data range of more negative values (i.e., northern latitudes) showed minimal variability, compared to less negative values (i.e., southern latitudes; Fig. 1). This supports other data that show predicting natal origins is more problematic in southerly latitudes with more positive isotopic values (Smith et al. 2009). Finally, unlike some raptors, such as accipiters and falcons, which may feed largely on migratory species, Golden Eagle prey species are generally much more localized. For example, largely nonmigratory species such as jackrabbits (Lepus spp.), snowshoe hares (Lepus americanus), marmots (Marmota spp.), ground squirrels (Spermophilopsis spp.), and ungulates would have isotopic values that more accurately reflect the location of juvenile Golden Eagle origin. As such, we reason that this technique may have more utility for Golden Eagles than some other raptor species.

Utilizing stable isotope analysis can be an important tool for: (1) helping link Golden Eagle migration count data with population origin and status; and (2) identifying important breeding areas of migratory Golden Eagles. Approximately two-thirds of the juvenile Golden Eagles we sampled were likely to have natal origins in and around the Yukon and Northwest Territories, Canada, to east and northeast Alaska, suggesting this area may be an important breeding area for migratory Golden Eagles utilizing the RMF. This is corroborated by multiple breeding studies (McIntyre 1995, Young et al. 1995, McIntyre and Adams 1999, McIntyre 2002, 2008).

We encourage researchers to incorporate stable-hydrogen isotope analysis during fall migration, wintering, and mortality studies on Golden Eagles. Widespread use has potential to greatly increase our understanding about the natal origins of migratory Golden Eagles which will aid in better interpretation of trends in Golden Eagle migration count data and population status throughout North America. In addition, employing this technique on juvenile Golden Eagles found as mortalities (e.g., on wind farms) would yield an estimation of natal origin, as well as providing important insight into whether mortalities are from local (i.e., sedentary) or migratory (i.e., northern) populations.

Acknowledgments

We thank the many people who helped in the field, notably A. Shreading, B. Bedrosian, R. Crandall, S. Fuller, S. Wilson, N. Nies-Nesmith, T. Veto, and V. Slabe. Additionally, we thank The Wildlife Research Institute for data collected at the Rogers Pass site in 2004 and 2005. Also, thanks to Montana Fish, Wildlife and Parks (FW&P), U.S. Forest Service, P. Shanley (Helena National Forest) and S. Kloetzel (The Nature Conservancy) for providing logistical support. Funding was provided by J. Sparks and Bureau of Land Management-Missoula, Montana, The Fledgling Fund, LCAO Foundation, M.J. Murdock Foundation, Maki Foundation, Mountaineers Foundation, Patagonia Foundation, and private individuals. B. Bedrosian and M. Seidensticker provided helpful comments on earlier versions of this manuscript. R. Corley helped with later versions of our map. This work was permitted under United States Department of the Interior, U.S. Geological Survey, Patuxent Wildlife Research Center, Bird Banding Laboratory permit 23353 and Montana FW&P permits 547 and 029. Finally, we would like to thank three anonymous reviewers and the editorial staff at JRR for their expertise, advice, and guidance.

Literature Cited

1.

P.H. Bloom 1987. Capturing and handling raptors. Pages 99–123 in B.A. Giron Pendleton B.A. Millsap K.W. Clineand D.A. Bird [Eds.], Raptor management techniques manual. National Wildlife Federation, Washington, DC U.S.A. Google Scholar

2.

P.H. Bloomand W.S. Clark 2001. Molt and sequence of plumage of Golden Eagles and a technique for in-hand ageing. North American Bird Bander 26:97–116. Google Scholar

3.

G.R. Bortolotti 1984. Age and sex size variation in Golden Eagles. Journal of Field Ornithology 55:54–66. Google Scholar

4.

G.J. Bowen L.I. Wassenaarand K.A. Hobson 2005. Global application of stable hydrogen and oxygen isotopes to wildlife forensics. Oecologia 143:337–348. Google Scholar

5.

C.P. Chamberlain J.D. Blum J.T. Holmes X. Feng T.W. Sherryand G.R. Graves 1997. The use of isotope tracers for identifying populations of migratory birds. Oecologia 109:132–141. Google Scholar

6.

J.P. DeLong T.D. Meehanand R.B. Smith 2005. Investigating fall movements of hatch-year Flammulated Owls (Otus flammeolus) in central New Mexico using stable hydrogen isotopes. Journal of Raptor Research 39:19–25. Google Scholar

7.

T.C. Edwards Jrand M.N. Kockert 1986. Use of body weight and length of footpad as predictors of sex in Golden Eagles. Journal of Field Ornithology 57:317–319. Google Scholar

8.

M.R. Fullerand J.A. Mosher 1981. Methods of detecting and counting raptors: a review. Pages 235–246 in C.J. Ralphand J.M. Scott [Eds.], Estimating the numbers of terrestrial birds. Studies in Avian Biology 6. Google Scholar

9.

K.A. Hobson 2005. Using stable isotopes to trace long-distance dispersal in birds and other taxa. Diversity and Distributions 11:157–164. Google Scholar

10.

K.A. Hobson H. Dement S.L. Van Milgenburgand L.I. Wassenaar 2009. Origins of American Kestrels wintering at two southern U.S. sites: an investigation using stable-isotope (δD,δO) methods. Journal of Raptor Research 43:325–337. Google Scholar

11.

K.A. Hobsonand L.I. Wassenaar 1997. Linking breeding and wintering grounds of neotropical migrant songbirds using stable hydrogen isotopic analysis of feathers. Oecologia 109:142–148. Google Scholar

12.

K.A. Hobsonand L.I. Wassenaar 1999. Stable isotope ecology: an introduction. Oecologia 120:312–313. Google Scholar

13.

S.W. Hoffmanand J.P. Smith 2003. Population trends of migratory raptors in western North America, 1977–2001. Condor 105:397–419. Google Scholar

14.

S.W. Hoffmanand J.P. Smith 2005. Do Golden Eagles warrant special concern based on migration counts in the western United States? Reply to McCaffery and McIntyre. Condor 107:473–475. Google Scholar

15.

J.F. Kelly V. Atudorei Z.D. Sharoand D.M. Finch 2002. Insights into Wilson's Warbler's migration from analyses of hydrogen stable-isotope ratios. Oecologia 130:216–221. Google Scholar

16.

J.F. Kelly K.C. Rueggand T.B. Smith 2005. Combining isotopic and genetic markers to identify breeding origins of migrant birds. Ecological Applications 15:1487–1494. Google Scholar

17.

M.N. Kochertand K. Steenhof 2002. Golden Eagles in the U.S. and Canada: status, trends, and conservation challenges. Journal of Raptor Research 36:32–40. Google Scholar

18.

M.N. Kochert C. McIntyreand E. Craig 2002. Golden Eagle (Aquila chrysaetos). In A. Pooleand F. Gill [Eds.], The Academy of Natural Sciences, Philadelphia, PA and the American Ornithologists’ Union, Washington, DC U.S.A. Google Scholar

19.

K.A. Lott T.D. Meehanand K.A. Heath 2003. Estimating the latitudinal origins of migratory birds using hydrogen and sulfur stable isotopes in feathers: influence of marine prey base. Oecologia 134:505–510. Google Scholar

20.

K.A. Lottand J.P. Smith 2006. A geographic-information system approach to estimating the origins of migratory raptors in North America using stable hydrogen isotope ratios in feathers. Auk 123:822–835. Google Scholar

21.

B.J. McCafferyand C.L. McIntyre 2005. Disparities between results and conclusion: do Golden Eagles warrant special concern based on migration counts in the western United States? Condor 107:469–473. Google Scholar

22.

C.L. McIntyre 1995. Nesting ecology of migratory Golden Eagles (Aquila chrysaetos) in Denali National Park, Alaska. M.S. thesis, Univ. of Alaska, Fairbanks, AK U.S.A. Google Scholar

23.

C.L. McIntyre 2002. Patterns in nesting area occupancy and reproductive success of Golden Eagles (Aquila chrysaetos) in Denali National Park and Preserve, Alaska. Journal of Raptor Research. 36 (1 Supplement). 50–54. Google Scholar

24.

C.L. McIntyre 2008. Movements of Golden Eagles (Aquila chrysaetos) from interior Alaska during their first year of independence. Auk 125:214–224. Google Scholar

25.

C.L. McIntyreand L.G. Adams 1999. Reproductive characteristics of migratory Golden Eagles in Denali National Park, Alaska. Condor 101:115–123. Google Scholar

26.

T.D. Meehan C.A. Lott Z.D. Sharp R.B. Smith R.N. Rosenfield A.C. Stewartand R.K. Murphy 2001. Using hydrogen isotope geochemistry to estimate the natal latitudes of immature Cooper’s Hawks migrating through the Florida Keys. Condor 103:11–20. Google Scholar

27.

T.D. Meehan R.N. Rosenfield V.N. Atudorei J. Bielefeldt L.J. Rosenfield AC. Stewart W.E. Stoutand M.A. Bozek 2003. Variation in hydrogen stable-isotope ratios between adult and nestling Cooper’s Hawks. Condor 105:567–572. Google Scholar

28.

J.E. Pagel D.M. Whittingtonand G.T. Allen 2010. Interim Golden Eagle inventory and monitoring protocols; and other recommendations. Division of Migratory Bird Management, U.S.D.I. Fish and Wildlife Service, Carlsbad, CA U.S.A. Google Scholar

29.

R.N. Rosenfield J. Bielefeldt R.K. Andersonand J.M. Patt 1991. Status reports: accipiters. Pages 42–49 in B.A. Giron Pendletonand D.L. Krahe [Eds.], Proceedings of the Midwest Raptor Symposium and Workshop. National Wildlife Federation, Washington, DC U.S.A. Google Scholar

30.

D.E. Rubenstein C.P. Chamberlin T. Holmes M.P. Ayeares J.R. Waldbaur G.R. Gravesand N.C. Tuross 2002. Linking breeding and wintering ranges of a migratory songbird using stable isotopes. Science 295:1062–1065. Google Scholar

31.

C.D. Ruyck K.A. Hobson N. Koper L.W. Larsonand L.I. Wassenaar 2013. An appraisal of the use of hydrogen isotope methods to delineate origins of migratory saw-whet owls in North America. Condor 115:366–374. Google Scholar

32.

P. Sherrington 2009. Piitaistakis-South Livingstone—2008. Final report. Rocky Mountain Eagle Research Foundation, Canmore, AB Canada. Google Scholar

33.

A.D. Smithand A.M. Dufty Jr 2005. Variation in the stable-hydrogen isotope composition of Northern Goshawk feathers: relevance to the study of migratory origins. Condor 107:547–558. Google Scholar

34.

A.D. Smith C.A. Lott J.P. Smith K.C. Donohue S. Wittenburg K.G. Smithand L. Goodrich 2009. Deuterium measurements of raptor feathers: does a lack of reproducibility compromise geographic assignment? Auk 126:41–46. Google Scholar

35.

J.P. Smith C.J. Farmer S.W. Hoffman G.S. Kaltenecker K.Z. Woodruffand P.F. Sherrington 2008. Trends in autumn counts of migratory raptors in western North America. State of North America’s birds of prey. Series in Ornithology 3:217–251. Google Scholar

36.

R.B. Smith T.D. Wolfand B.O. Meehan 2003. Assessing migration patterns of Sharp-shinned Hawks Accipiter striatus using stable-isotope and band encounter analysis. Journal of Avian Biology 34:387–392. Google Scholar

37.

F. Tilly 2008. HMANA western mountain flyway report. Hawk Migration Studies 24:47–56. Google Scholar

38.

K. Titusand M.R. Fuller 1990. Recent trends in counts of migrant hawks from northeastern North America. Journal of Wildlife Management 54:463–470. Google Scholar

39.

S.L. VanWilgenburgand K.A. Hobson 2011. Combining stable-isotope (δD) and band recovery data to improve probabilistic assignment of migratory birds to origin. Ecological Applications 21:1340–1351. Google Scholar

40.

C. Viverette L.J. Goodrichand K.L. Bildstein 1996. Decreases in migrating Sharp-shinned Hawks (Accipter striatus) at traditional raptor migration watch sites in eastern North America. Auk 133:32–40. Google Scholar

41.

L.I. Wassenaar 2008. An introduction to light stable isotopes for use in terrrestrial animal migration studies. Pages 21–44 in K.A. Hobsonand L.I. Wassenaar [Eds.], Tracking animals using stable hydrogen isotopes. Academic Press, New York, NY U.S.A. Google Scholar

42.

L.I. Wassenaarand K.A. Hobson 2000a. Stable-carbon and hydrogen isotope ratios reveal breeding origins of Red-winged Blackbirds. Ecological Applications 10:911–916. Google Scholar

43.

L.I. Wassenaarand K.A. Hobson 2000b. Improved method for determining the stable-hydrogen isotopic composition (δD) of complex organic materials of environmental interest. Environmental Science Technology 34:2354–2360. Google Scholar

44.

L.I. Wassenaarand K.A. Hobson 2003. Comparative equilibration and online technique for determination of non-exchangeable hydrogen of keratins for use in animal migration studies. Isotopes in Environmental and Health Studies 39:211–217. Google Scholar

45.

L.I. Wassenaarand K.A. Hobson 2006. Stable-hydrogen isotope heterogeneity in keratinous materials: mass spectrometry and migratory wildlife tissue subsampling strategies. Rapid Communications in Mass Spectrometry 20:2205–2510. Google Scholar

46.

S.R. Wittenberg S.E. Lehnenand K. Smith 2013. Use of stable hydrogen to predict natal origins of juvenile Merlins and Northern Harriers migrating through the Florida Keys. Condor 115:451–455. Google Scholar

47.

M.K. Wunder A. Hobson J. Kelly P. Marra L.I. Wassenaar C. Strickerand R. Doucette 2009. Does a lack of design and repeatability compromise scientific criticism? A response to Smith et al. Auk 126:922–926. Google Scholar

48.

D.D. Young Jr C.L. McInyre P.J. Bente T.R. McCabeand R.E. Ambrose Jr 1995. Nesting by Golden Eagles on the north slope of the Brooks Range in northeastern Alaska. Journal of Field Ornithology 66: 373–379. Google Scholar
© 2015 The Raptor Research Foundation, Inc.
Robert Domenech, Tim Pitz, Kathy Gray, and Melanie Smith "Estimating Natal Origins of Migratory Juvenile Golden Eagles Using Stable Hydrogen Isotopes," Journal of Raptor Research 49(3), 308-315, (1 September 2015). https://doi.org/10.3356/JRR-14-19.1
Received: 28 February 2014; Accepted: 20 January 2015; Published: 1 September 2015
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