Altay Prefecture plays a vital role as an ecological barrier in Northwest China. Studying the ecosystem service value is of great significance for promoting regional green high-quality development and maintaining ecological security. Based on Global ESA land cover data from 2000 to 2015, the trade-off and synergy relationships and driving force factors between ecosystem services in Altay Prefecture were analyzed in this study. The analysis produced four main results. (1) The ecosystem service value in Altay Prefecture continued to increase from 113.521 × 10⁹ yuan in 2000 to 115.777 × 10⁹ yuan in 2015, for an increase of about 1.98%. (2) The distribution of ecosystem service value had obvious spatial agglomeration characteristics, with hot spot areas mainly concentrated in the ″two rivers and one lake″ and the mountainous areas in the northwest, while the cold spot areas were mainly the forest and grass-covered areas in the northern mountainous areas and within Jimunai County. (3) The trade-off and synergy relationship among ecosystem services was mainly synergistic, with a total of 77.78% of ecosystem service relative relationships showing a significant positive correlation at the 0.01 level. (4) Economic factors and industrial structure are important factors affecting ecosystem service value in Altay Prefecture. Ecosystem service value is positively correlated with per capita GDP and the output value of the tertiary industry, but negatively correlated with the output value of the secondary industry.