Field work to survey and investigate the Anhui Yaoluoping Macrofungi Nature Reserve was undertaken in order to understand and rationally develop macrofungi resources. The results showed that there are, in total, 101 macro-fungal species and varieties, including 59 kinds of edible mushroom, 20 kinds of medicinal mushroom, and 8 kinds of toadstools, grouped according to their economic values. The species belong to 5 classes, 13 orders, 43 families and 67 genera, Another 14 species are unknown fungus food or medicine and remains to be further developed. They are widely distributed in broad-leaved forests, mixed forests, coniferous forests, bamboo forests, wastelands and areas with other vegetation types. Large fungi resources are abundant; some varieties are rare and very valuable to develop. Among those of value are some edible and medicinal mushrooms, anti-cancer sulfur bacteria, morchella, gold equivalent cow bacilli, pine mushrooms, etc. Some species and species resources have still not been fully investigated. These large fungi can be domesticated and cultivated, and have very good commercial development prospects.