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1 August 2006 TISSUE DISTRIBUTION AND HEMOLYMPH ACTIVITY OF SIX ENZYMES IN THE AMERICAN LOBSTER (HOMARUS AMERICANUS): POTENTIAL MARKERS OF TISSUE INJURY
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Abstract

Biochemistry panels are used to help identify tissue injury (e.g., because of inflammation, trauma or hypoxia) in human and veterinary medicine in part, by detecting increased enzyme activity in serum or plasma after release from damaged tissues. To determine if a similar approach can be used in Homarus americanus, activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutamate dehydrogenase (GD), sorbitol dehydrogenase (SDH), amylase (AMY) and lipase (LIP) were measured in tissue homogenates of heart, hepatopancreas, abdominal muscle, proximal intestine, antennal gland, hemocyte lysate supernatant and hemolymph plasma and serum. Activities of ALT and AST were significantly higher in serum than plasma, which was attributed to release of enzymes from hemocytes during coagulation. Reference intervals calculated for plasma enzyme activity at ambient holding temperatures of 2°C to 4°C and 15°C were quite similar. Plasma enzyme activity was not a sensitive test for detecting infection with Aerococcus viridans (gaffkemia) during an experimental trial.

ANDREA BATTISON "TISSUE DISTRIBUTION AND HEMOLYMPH ACTIVITY OF SIX ENZYMES IN THE AMERICAN LOBSTER (HOMARUS AMERICANUS): POTENTIAL MARKERS OF TISSUE INJURY," Journal of Shellfish Research 25(2), 553-560, (1 August 2006). https://doi.org/10.2983/0730-8000(2006)25[553:TDAHAO]2.0.CO;2
Published: 1 August 2006
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