The presence of the lipophilic toxins okadaic acid (OA), dinophysistoxin-1, pectenotoxin-2, and yesso toxin (YTX) in the abalone Haliotis discus hannai collected between March 2009 and May 2010 was analyzed via liquid chromatographytandem mass spectrometry (LC-MS/MS) and the mouse bioassay. Thirty abalone samples collected from 2 sites, Geumdang and Chungdo on the southwest coast of Korea, were submitted for toxin analysis. Lipophilic toxins OA and YTX were detected in the digestive gland of the abalone by LC-MS/MS, but no toxins were detected in the foot muscle. The highest toxin concentrations in the digestive glands of the abalone were 4.7 ng/g OA and 1.3 ng/g YTX. Okadaic acid and YTX were detected mainly during winter and spring. However, dinophysistoxin-1 and pectenotoxin-2 were not detected in any of the samples by LC-MS/MS. Diarrhetic shellfish poisoning toxins were not detected in the digestive gland or the foot muscle by mouse bioassay. Although low levels of toxins were detected, this is the first report identifying lipophilic toxins in abalone.