Insecticide resistance to organophosphates and carbamates can be the result of changes in acetylcholinesterase activity conferred by the ACE-1 mutation. Detection of this altered target site mutation is important in guiding informed decisions for resistance management. In this study we compared a competitive enzyme assay with a polymerase chain reaction assay utilizing a restriction enzyme. Both assays detected the ACE-1 mutation in Culex quinquefasciatus and agreement was 100%. The costs and benefits of each assay are presented.
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1 December 2012
Comparison of Biochemical and Molecular Tests for Detecting Insecticide Resistance Due to Insensitive Acetylcholinesterase in Culex quinquefasciatus
Mariah L. Scott,
Janet C. McAllister
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ACE-1 mutation
acetylcholinesterase
Culex quinquefasciatus
organophosphate resistance