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1 December 2016 Genetic Polymorphism of Aedes albopictus Population Inferred From ND5 Gene Variabilities In Subang Jaya, Malaysia
Nurul Adilah-Amrannudin, Mayamin Hamsidi, Nurul-Ain Ismail, Rodziah Ismail, Nazri Che Dom, Abu Hassan Ahmad, Mohd Fahmi Mastuki, Tengku Shahrul Anuar Tengku Ahmad Basri, Adira Khalid, Mohammad Muslim, Nurul Amalina Ahmad Daud, Siti Nazrina Camalxaman
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Abstract

This study was performed to establish the genetic variability of Aedes albopictus within Subang Jaya, Selangor, Malaysia, by using the nicotinamide adenine dinucleotide dehydrogenase 5 subunit (ND5) mitochondrial DNA (mtDNA) marker. A total of 90 samples were collected from 9 localities within an area of the Subang Jaya Municipality. Genetic variability was determined through the amplification and sequencing of a fragment of the ND5 gene. Eight distinct mtDNA haplotypes were identified. The evolutionary relationship of the local haplotypes alongside 28 reference strains was used to construct a phylogram, the analysis of which revealed low genetic differentiation in terms of both nucleotide and haplotype diversity. Bayesian method was used to infer the phylogenetic tree, revealing a unique relationship between local isolates. The study corroborates the reliability of ND5 to identify distinct lineages for polymorphism-based studies and supplements the existing body of knowledge regarding its genetic diversity. This in turn could potentially aid existing vector control strategies to help mitigate the risk and spread of the dengue virus.

Copyright © 2016 by The American Mosquito Control Association, Inc.
Nurul Adilah-Amrannudin, Mayamin Hamsidi, Nurul-Ain Ismail, Rodziah Ismail, Nazri Che Dom, Abu Hassan Ahmad, Mohd Fahmi Mastuki, Tengku Shahrul Anuar Tengku Ahmad Basri, Adira Khalid, Mohammad Muslim, Nurul Amalina Ahmad Daud, and Siti Nazrina Camalxaman "Genetic Polymorphism of Aedes albopictus Population Inferred From ND5 Gene Variabilities In Subang Jaya, Malaysia," Journal of the American Mosquito Control Association 32(4), 265-272, (1 December 2016). https://doi.org/10.2987/16-6579.1
Published: 1 December 2016
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KEYWORDS
Aedes albopictus
genetic variation
haplotype
nicotinamide adenine dinucleotide dehydrogenase 5 subunit
phylogenetic tree
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