We record 16 species of spider wasps from Puerto Rico. One new species, Auplopus taino (Pepsinae), is described from a single female. The types of the long unrecognized Pompilus cressoni Dewitz, 1881 have been examined and found to be a species of Episyron, a genus not previously reported from Puerto Rico; E. cressoni is a senior synonym of E. conterminus posterus (W. Fox, 1893), a common North American species. Batazonus hookeri and B. mundiformis, now placed in Poecilopompilus and both described by Rohwer, 1915, are opposite sexes of a single taxon; P. hookeri has page priority. We here treat this wasp as a subspecies of the widespread Neotropical species, P. flavopictus (F. Smith, 1862).

Keys in both English and Spanish are provided for the separation of the known species, as are appropriate illustrations.