The leafhoppers of the subfamily Gyponinae are reviewed for the island of Hispaniola. Prior to this study the subfamily was known from five species of Curtara. The present study adds three genera (Prairiana, Rugosana, and Gypona) and 16 species, with 14 of these described as new. Therefore, there are four genera and twenty one species now known from Hispaniola. They are as follows: Prairiana dualis DeLong, Curtara (Curtara) cavera DeLong and Freytag, C. (C.) sata DeLong and Freytag, C. (C.) carloorum Freytag and Carlo, C. (C.) acroschismata Freytag and Carlo, C. (C.) blancoi Freytag and Carlo, C. (C.) albimaculata (Osborn), C. (C.) barrera Freytag, n. sp., C. (C.) youngi Freytag, n. sp., C. (C.) minima Freytag, n. sp., C. (C.) cornuta Freytag, n. sp. C. (C.) nunezi Freytag, n. sp., Rugosana carpa Freytag, n. sp., R. reta Freytag, n. sp., Gypona (Obtusana) curta Freytag, n. sp., G. (O.) ileota Freytag, n. sp., G. (O.) imita Freytag, n. sp., G. (O.) toxum Freytag, n. sp. G. (Marganalana) woodruffi Freytag, n. sp., G. (M.) rawlinsi Freytag, n. sp. and G. (M.) spina Freytag, n. sp.