A survey of Formicidae was conducted in the Tombigbee National Forest (Ackerman Unit) in Winston and Choctaw Counties, Mississippi. Collections were made with pitfall traps from 15 March 1999 to 6 March 2000 and with other collecting methods on various dates from the spring of 2002 through the fall of 2004. A total of 27 genera and 72 species representing 7 subfamilies are reported from the Tombigbee National Forest. Seven new state records are given, as well as records of several rare and uncommon species. Habitat and microhabitat information are given for each species.