Agroforestry systems are widely recognized for its provision of ecosystem services including but not limited to provisional, regulatory, cultural and supporting services. Apart from the monetary benefits, it plays an important role in biodiversity augmentation and conservation. The paper aims to assess the impact of multifunctional agroforestry on butterfly biodiversity that was established in 0.75 acres with 24 different tree species and 8 intercrops organized into four equally sized, distinct quadrats at Forest College and Research Institute, Mettupalayam. The butterflies were counted and observed by Pollard walk method on alternate days in three different time slots (09:00-10:00 hours, 12:00-13:00 hours and 15:00-16:00 hours) for three months (December, 2023 to February, 2024). A total of 42 species of butterflies belonging to four families were recorded during the study period. Among the four families, maximum numbers were found in Nymphalidae (45%), Pieridae (26%), Papilonidae (17%) and Lycaenidae (12%). The relative abundance, species richness, diversity (H'), dominance (D), evenness (E) and equitability (J) index were calculated in each quadrat at different time slots and were found to be significantly different. Owing to its highest species richness and diversity index, Quadrat I was considered to be a better habitat for butterflies compared to other quadrats (H'=3.46; D=0.037; E=0.80; J=0.938; Abundance= 31%).