An examination of leaf beetle specimens (Coleoptera: Chrysomelidae) in the largest beetle collections in Kentucky, recent inventory work in state nature preserves and other protected areas, and a review of the literature revealed thirty species of the tribes Galerucini and Luperini (Subfamily Galerucinae) present in Kentucky, thirteen of which are previously unreported for the state. Distribution maps and label data are presented for the thirty Kentucky species including spatial (state and Kentucky county records), temporal (years and months of collection in Kentucky), and plant association information. The following species are reported from Kentucky for the first time: Derospidea brevicollis (LeConte), Monocesta coryli (Say), Trirhabda canadensis (Kirby), Galerucella nymphaeae (L.), Ophraella americana (F.), Ophraella cribrata (LeConte), Acalymma gouldi Barber, Acalymma vinctum (LeConte), Phyllecthris dorsalis (Olivier), Phyllecthris gentilis LeConte, Phyllobrotica limbata (F.), Phyllobrotica stenidea Schaeffer, and Metroidea brunnea (Crotch).