An examination of leaf beetle specimens (Coleoptera: Chrysomelidae) in the largest beetle collections in Kentucky, recent inventory work in state nature preserves and other protected areas, and a review of the literature revealed forty-six species of the subfamily Eumolpinae present in Kentucky, twenty of which previously were unreported for the state. Distribution maps and label data are presented for the forty-six Kentucky species, including spatial (state and Kentucky county records), temporal (years and months of collection in Kentucky), and plant association information. The following species are reported from Kentucky for the first time: Metachroma carolinense Blake, Metachroma laterale Crotch, Metachroma orientale Blake, Graphops curtipennis curtipennis (F. E. Melsheimer), Graphops marcassita marcassita (Crotch), Graphops simplex J. L. LeConte, Graphops varians J. L. LeConte, Paria fragariae Wilcox, Paria pratensis Balsbaugh, Paria sellata (Horn), Colaspis favosa Say, Colaspis suilla suilla F., Glyptoscelis pubescens (F.), Spintherophyta globosa (Olivier), Tymnes metasternalis (Crotch), Tymnes violaceus Horn, Demotina modesta Baly, Fidia longipes (F. E. Melsheimer), Xanthonia angulata Staines & Weisman, and Xanthonia serrata Staines & Weisman.