An examination of leaf beetle specimens (Coleoptera: Chrysomelidae) in the largest beetle collections in Kentucky, recent inventory work in state nature preserves and other protected areas, and a review of the literature revealed 59 species of Cryptocephalinae present in Kentucky, 27 of which were previously unreported for the state. Distribution maps and label data are presented for the 59 Kentucky species, including spatial (state and Kentucky county records), temporal (years and months of collection in Kentucky), and plant association information. The following species are reported from Kentucky for the first time: Griburius scutellaris (F.), Pachybrachis bivittatus (Say), Pachybrachis confusus Bowditch, Pachybrachis diversus Fall, Pachybrachis hepaticus hepaticus (F. E. Melsheimer), Pachybrachis luridus (F.), Pachybrachis morosus Haldeman, Pachybrachis obsoletus Suffrian, Pachybrachis othonus othonus (Say), Pachybrachis peccans Suffrian, Pachybrachis pectoralis (F. E. Melsheimer), Pachybrachis praeclarus Weise, Pachybrachis spumarius Suffrian, Pachybrachis trinotatus (F. E. Melsheimer), Pachybrachis viduatus (F.), Bassareus lituratus (F.), Cryptocephalus calidus Suffrian, Cryptocephalus fulguratus J. L. LeConte, Cryptocephalus gibbicollis decrescens R. White, Cryptocephalus mutabilis F. E. Melsheimer, Cryptocephalus notatus F., Cryptocephalus striatulus J. L. LeConte, Diachus catarius (Suffrian), Diachus chlorizans (Suffrian), Triachus atomus (Suffrian), Coleopthorpa dominicana franciscana (J. L. LeConte), and Neochlamisus gibbosus (F.).