Kentucky's crayfish faunal list has undergone considerable changes since the publication of Crayfishes of Kentucky (Taylor and Schuster, 2004). We conducted an in-depth literature review and reviewed collection databases to develop an up-to-date checklist of crayfish species known to occur in Kentucky. Sixty-five crayfish species have been documented from Kentucky to date, including twelve new species described and three state records since 2004. One new species description (Faxonius sp. A. nov. sp.) is currently underway and was included in the species list for future reference. Additionally, we provide information on changes in crayfish taxonomy, crayfish species that may occur in Kentucky, and potential cryptic species where additional research may result in new species being detected. We hope this information provides clarity for researchers and resource managers and encourages additional crayfish research in Kentucky.