Ten gray foxes, eight principals that were fed approximately 4.4 × 10¹⁰ colony forming units of Brucella abortus strain 2308 and two controls, were examined for serologic responses and tissue distribution of the organisms. Blood sera from each fox were tested on the day of exposure and at seven weekly intervals for antibodies to B. abortus, using the brucellosis card, standard tube agglutination, 2-mercaptoethanol and rivanol tests. Control foxes were serologically negative for all tests throughout the study and the principals were negative prior to exposure. On days 14, 21 and 28, the eight principals had positive card reactions and ≥1:100 tube agglutination titers. After 28 days, the titers receded; and by day 49, three principals had negative card reactions and one of these was negative for all tests. Brucella abortus was isolated from one or more lymph nodes from seven of eight principals including the one which was seronegative. The bacterium was not isolated from lungs, livers, spleens, kidneys, uteri or testicles.