Two hundred eighteen usable serum samples were collected from hunter-killed collared peccaries (Tayassu tajacu) during March 1986, in three areas of Arizona. Evaluations for antibodies against vesicular stomatitis virus (VSV) New Jersey (NJ) type, VSV Indiana type, pseudorabies virus, brucellosis, and leptospirosis revealed positive test results in 8%, 0%, <1%, 0%, and 23% of the sera, respectively. Exposure of peccaries to VSV (NJ) was widespread, but variation in the prevalence of seropositive peccaries was not found between the three areas sampled. The exposure of peccaries to VSV (NJ) probably was related to the recent epizootics in livestock in the vicinity. Exposure to Leptospira interrogans serovars also was widespread, and geographic variation in the prevalence of peccaries with antibodies against L. interrogans was found.