A combination of 100 mg ketamine hydrochloride (KH) and 20 mg xylazine hydrochloride (XH) was used to immobilize fishers (Martes pennanti). Four adult males were intramuscularly injected a total of five times at dosages between 22.4 to 29.0 mg/kg KH and 4.1 to 6.6 mg/kg XH. Mean (±SE) induction time and arousal time were 3.3 ± 0.5 min and 76.8 ± 12.1 min, respectively. Respiration, heart rate, and body temperature in response to sedation appeared normal. A 5:1 mixture of KH-XH appears to be a safe immobilizing agent for fishers.