Three Tsushima leopard cats (Felis bengalensis euptilura) in Japan were collected as road kills. Three species of trematodes (Pharyngostomum cordatum, Paragonimus sp. and Dicrocoeliidae gn. sp.), one species of cestode (Spirometra erinacei) and nine species of nematodes (Arthrostoma hunanensis, Uncinaria felidis, Uncinaria sp., Ancylostoma tubaeforme, Molineus springsmithi, Toxocara cati, Capillaria aerophila, Capillaria feliscati, and Capillaria sp.) were found. Among these helminths, Arthrostoma hunanensis, Uncinaria spp., and Molineus springsmithi have not been reported previously in Japanese domestic cats.