Pulmonary cryptococcosis was observed in a free-living adult female common toad (Bufo bufo) that was killed by a vehicle. Both lungs had various eosinophilic, monomorphic, and spherical to elliptical organisms identified as Cryptoccocus spp. The yeasts were demonstrated by Grocott's silver method and the periodic acid-Schiff reaction and the capsule was positive for mucin with a mucicarmine stain. The agent was confirmed by immunohistochemistry, using the monoclonal antibody anti-Cryptococcus neoformans, and by a polymerase chain reaction-based method using a C. neoformans-specific primer. This report, to the best of our knowledge, represents the first case of cryptococcosis in a common toad.
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1 April 2008
A Case of Pulmonary Cryptococcosis in a Free-living Toad (Bufo bufo)
Fernanda Seixas,
Maria da Luz Martins,
Maria de Lurdes Pinto,
Paulo Jorge Travassos,
Manuela Miranda,
Maria dos Anjos Pires
Journal of Wildlife Diseases
Vol. 44 • No. 2
April 2008
Vol. 44 • No. 2
April 2008
Bufo bufo
cryptococcosis
Cryptococcus neoformans
fungal diseases
toad