Papillomatous skin lesions from a green lizard (Lacerta viridis) were examined histologically, using electron microscopy and DNA was isolated from the lesions for herpesviral DNA detection. Histology confirmed the lesions to be squamous epithelial papillomas. Using electron microscopy, no virus particles were detected. The specific sequence of herpesviral DNA-directed DNA polymerase (EC 2.7.7.7) was amplified using degenerate primers in a nested format. The 235–base-pair (bp) sequence was sequenced and compared with previously published DNA-directed DNA polymerase sequences from various reptile herpesviruses. The sequence from the green lizard showed significant similarity with sequence of fibropapilloma-associated turtle herpesviruses from sea turtles.
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1 January 2010
Herpesvirus-Associated Papillomatosis in a Green Lizard
I. Literak,
B. Robesova,
V. Majlathova,
I. Majlath,
P. Kulich,
P. Fabian,
E. Roubalova
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Journal of Wildlife Diseases
Vol. 46 • No. 1
January 2010
Vol. 46 • No. 1
January 2010
DNA-directed DNA polymerase
electron microscopy
green lizard
herpesvirus
Hungary
Lacerta viridis
papilloma