Use of Real-time PCR to Detect Canine Parvovirus in Feces of Free-ranging Wolves

L. David Mech; Emily S. Almberg; Douglas Smith; Sagar Goyal; Randall S. Singer

doi:10.7589/0090-3558-48.2.473

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L. David Mech,^1,* Emily S. Almberg,² Douglas Smith,³ Sagar Goyal,⁴ Randall S. Singer⁴

¹1 US Geological Survey, Northern Prairie Wildlife Research Center, 8711 37th St. SE, Jamestown, Nort
²2 Department of Fisheries, Wildlife and Conservation Biology, University of Minnesota, St. Paul, Min
³3 Yellowstone Center for Resources, Yellowstone National Park, Wyoming 82190, USA
⁴4 College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota 55108, USA

^* Corresponding author (email: david_mech@usgs.gov)

Abstract

Using real-time PCR, we tested 15 wolf (Canis lupus) feces from the Superior National Forest (SNF), Minnesota, USA, and 191 from Yellowstone National Park (YNP), USA, collected during summer and 13 during winter for canine parvovirus (CPV)-2 DNA. We also tested 20 dog feces for CPV-2 DNA. The PCR assay was 100% sensitive and specific with a minimum detection threshold of 10⁴ 50% tissue culture infective dose. Virus was detected in two winter specimens but none of the summer specimens. We suggest applying the technique more broadly especially with winter feces.

Citation Download Citation

L. David Mech, Emily S. Almberg, Douglas Smith, Sagar Goyal, and Randall S. Singer "Use of Real-time PCR to Detect Canine Parvovirus in Feces of Free-ranging Wolves," Journal of Wildlife Diseases 48(2), 473-476, (1 April 2012). https://doi.org/10.7589/0090-3558-48.2.473

Received: 9 May 2011; Accepted: 1 November 2011; Published: 1 April 2012

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