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1 January 2013 ISOLATION AND CHARACTERIZATION OF A NOVEL HERPESVIRUS FROM A FREE-RANGING EASTERN GREY KANGAROO (MACROPUS GIGANTEUS)
Paola Karinna Vaz, Julian Motha, Christina McCowan, Nino Ficorilli, Pam Lizette Whiteley, Colin Reginald Wilks, Carol Anne Hartley, James Rudkin Gilkerson, Glenn Francis Browning, Joanne Maree Devlin
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Abstract

We isolated a macropodid herpesvirus from a free-ranging eastern grey kangaroo (Macropus giganteous) displaying clinical signs of respiratory disease and possibly neurologic disease. Sequence analysis of the herpesvirus glycoprotein G (gG) and glycoprotein B (gB) genes revealed that the virus was an alphaherpesvirus most closely related to macropodid herpesvirus 2 (MaHV-2) with 82.7% gG and 94.6% gB amino acid sequence identity. Serologic analyses showed similar cross-neutralization patterns to those of MaHV-2. The two viruses had different growth characteristics in cell culture. Most notably, this virus formed significantly larger plaques and extensive syncytia when compared with MaHV-2. No syncytia were observed for MaHV-2. Restriction endonuclease analysis of whole viral genomes demonstrated distinct restriction endonuclease cleavage patterns for all three macropodid herpesviruses. These studies suggest that a distinct macropodid alphaherpesvirus may be capable of infecting and causing disease in eastern grey kangaroos.

Paola Karinna Vaz, Julian Motha, Christina McCowan, Nino Ficorilli, Pam Lizette Whiteley, Colin Reginald Wilks, Carol Anne Hartley, James Rudkin Gilkerson, Glenn Francis Browning, and Joanne Maree Devlin "ISOLATION AND CHARACTERIZATION OF A NOVEL HERPESVIRUS FROM A FREE-RANGING EASTERN GREY KANGAROO (MACROPUS GIGANTEUS)," Journal of Wildlife Diseases 49(1), 143-151, (1 January 2013). https://doi.org/10.7589/2012-01-027
Received: 20 January 2012; Accepted: 1 August 2012; Published: 1 January 2013
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