During the last three decades, much research has illustrated the potential impact of diseases on wild animal populations. However, few studies focused on establishing parameters to reliably differentiate healthy from sick animals. We present clinical, hematologic, and serum chemistry data from nine healthy ocelots (Leopardus pardalis) and one ill ocelot captured in 2012 and 2013 in Brazil. Laboratory results were compared to mean values of captive ocelots and reference ranges for domestic cats (Felis catus). Free-ranging ocelots had overall higher white blood cells, aspartate aminotransferase, creatine phosphokinase, lactate dehydrogenase, urea nitrogen, and triglycerides, probably due to handling. Free-ranging ocelots also presented hyperproteinemia, hyperglobulinemia, hypoalbuminemia, and hyperuricemia.