The presence of antimicrobial-resistant bacteria in feces of 42 Peruvian Boobies (Sula variegata) from a Northern Peru island was evaluated using MicroScan and disk diffusion. Fourteen microorganisms were recovered, including three Pseudomonas spp. resistant to one antibiotic each, and four multiresistant Escherichia coli. Antimicrobial-resistant bacteria are reported in S. variegata.