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21 April 2022 Effect of Canine Adipose Mesenchymal Stem Cell Secretome on a Model of Second-Intention Wound Healing in the Red-Eared Slider Turtle (Trachemys scripta)
Sayda Pérez-Delgado, Pedro J. Ginel, Rafael Guerra, Elena Mozos, Cristina Alcoholado, María del Carmen Martin-Astorga, José Becerra, Antonio J. Villatoro
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Abstract

Mesenchymal stem cell (MSC) secretome refers to a variety of bioactive compounds that represents the more important pathway by which MSCs participate in tissue regeneration. Many of these compounds have shown variable functional activity even across nonmammalian vertebrate species, although MSCs in turtles have not yet been described. Canine adipose MSC secretome has been successfully used experimentally in skin healing. Our aim was to conduct a blinded controlled study to evaluate the effect of canine adipose MSC secretome (cS-MSC) as an alternative for the healing of soft skin, second intention wounds of red-eared slider turtles (Trachemys scripta). Under general anesthesia, one circular, 6-mm full thickness wound was made in each rear leg of 14 females. After randomization, cS-MSC was injected subcutaneously around one wound at days 1, 7, and 14, whereas the other wound acted as control. Biopsies from three animals' wounds were obtained at days 21, 28, 42, and 63. Differences in mean wound retraction at days 21 (n=14) and 28 (n=11) were statistically nonsignificant. The clinical and histopathologic scores performed blind by two different investigators were similar for treated and control wounds. In conclusion, we could not detect a significant functional activity of cS-MSC on wound healing of Trachemys scripta.

© Wildlife Disease Association 2022
Sayda Pérez-Delgado, Pedro J. Ginel, Rafael Guerra, Elena Mozos, Cristina Alcoholado, María del Carmen Martin-Astorga, José Becerra, and Antonio J. Villatoro "Effect of Canine Adipose Mesenchymal Stem Cell Secretome on a Model of Second-Intention Wound Healing in the Red-Eared Slider Turtle (Trachemys scripta)," Journal of Wildlife Diseases 58(2), 368-372, (21 April 2022). https://doi.org/10.7589/JWD-D-20-00100
Received: 5 June 2020; Accepted: 20 July 2021; Published: 21 April 2022
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KEYWORDS
mesenchymal stem cell
secretome
Trachemys scripta
wound healing
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