Serum samples from 1,544 white-tailed deer (Odocoileus Virginianus) collected in nine southeastern states were examined for leptospiral antibodies. Significant titers of 1:100 or greater were found in 292 deer. The highest prevalence of leptospiral antibodies was in Virginia, where 108 of 351 deer had significant titers. The most frequently encountered serotypes of Leptospira interrogans were: grippotyphosa (210 positive), pomona (81), and canicola (26). Other serotypes disclosed were austral is (15), icterohaemorrhagiae (10), pyrogenes (6), tarassovi (hyos) (5), georgia (4), ballum (4), sejroe (3), bataviae (2) and autumnalis (2).