Two laparoscopic ovariohysterectomies and three laparoscopic hysterectomies were performed on normal, healthy, adult African lions (Panthera leo) in dorsal recumbency, with the body tilted at 25° with the head down. One 12-mm trocar and two 5-mm trocars were used to access the uterus and ovaries, and the UltraCision®(Harmonic Scalpel® clamp or coagulation shears was used to coagulate and divide the ovarian- and the uterine-supporting structures and the uterine body. The animals recovered uneventfully from anesthesia and were released to their exhibits within 5–10 days of surgery. Such procedures can be performed safely on large felids and can reduce postoperative recovery time and postoperative complications. The scalpel facilitated the procedure by coagulating and dividing tissue in a continuous sequence and reducing the number of instruments required.