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1 June 2006 GIARDIA AND CRYPTOSPORIDIUM SPECIES AND GENOTYPES IN COYOTES (CANIS LATRANS)
James M. Trout, Mónica Santín, Ronald Fayer
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Abstract

Feces and duodenal scrapings were collected from 22 coyotes (Canis latrans) killed in managed hunts in northeastern Pennsylvania. Polymerase chain reaction (PCR) methods were used to detect Giardia and Cryptosporidium spp. PCR-amplified fragments of Giardia and Cryptosporidium spp. SSU-rRNA genes were subjected to DNA sequence analysis for species/genotype determination. Seven coyotes (32%) were positive for G. duodenalis: three assemblage C, three assemblage D, and one assemblage B. Six coyotes (27%) were positive for Cryptosporidium spp. One isolate shared 99.7% homology with C. muris, whereas five others (23%) shared 100% homology with C. canis, coyote genotype. This is the first report on multiple genotypes of Giardia spp. in coyotes and on the prevalence of Cryptosporidium spp. genotypes in coyotes.

James M. Trout, Mónica Santín, and Ronald Fayer "GIARDIA AND CRYPTOSPORIDIUM SPECIES AND GENOTYPES IN COYOTES (CANIS LATRANS)," Journal of Zoo and Wildlife Medicine 37(2), 141-144, (1 June 2006). https://doi.org/10.1638/05-06TYM-123005.1
Received: 30 December 2005; Published: 1 June 2006
KEYWORDS
Canis latrans
coyote
Cryptosporidium
genotypes
Giardia
prevalence
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