Twenty-two free-ranging golden jackals (Canis aureus) were immobilized with a combination of 113 ± 24 μg/kg medetomidine and 2.1 ± 0.3 mg/kg ketamine (M–K) or 88 ± 16 μg/kg medetomidine and 0.47 ± 0.08 mg/ kg midazolam (M–M) by i.m. injection. Induction and recovery times were recorded. Pulse rate, respiratory rate, body temperature, systolic and diastolic blood pressures, and oxygen saturation were measured. Anesthesia depth indicators were observed. There was no significant difference between the M–K and the M–M groups regarding induction time (6:14 ± 1:45 and 7:16 ± 2:09 min, respectively). Both combinations provided safe and effective immobilization for at least 20–30 min. Pulse rate was significantly higher in the M–K group. There was no significant difference in any other objective or subjective parameter. Following administration of atipamezole at five times the dose of medetomidine given, there was a significant difference between the two combinations in recovery time; M–K jackals were standing within 3:42 ± 2:17 min and M–M jackals within 8:47 ± 4:32 min. Neither of the combinations caused rough or prolonged recovery. Subjectively, the M–M group had smoother and less ataxic recovery.