A 13-mo-old, captive-born, female African wild dog (Lycaon pictus) was presented with a progressively enlarging, firm mass on the dorsum of the head. Due to rapid tumor growth and the development of neurologic signs, elective euthanasia and a complete postmortem were performed 2 months after initial presentation. Histologic findings were similar in both antemortem biopsy and postmortem samples from the tumor and consisted of nodules of cartilage and/or bone separated by thin interconnected trabeculae that contained spindle-shaped cells, consistent with multilobular tumor of bone. Very few reports of tumors in the endangered African wild dog have been published to date. To the authors' knowledge, this is the first report of an osseous tumor in an African wild dog and the first report of a multilobular tumor of bone in a nondomestic canid.