The objectives of this study were to optimize nested polymerase chain reaction (PCR) for Mycobacterium avium complex and Mycobacterium tuberculosis complex and apply them on samples from parrots. Results were negative for the presence of these Mycobacterium in the samples, and nested PCR was specific, faster, and more sensitive than other tests, thereby justifying its use in antemortem diagnosis.
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1 March 2014
OPTIMIZED NESTED POLYMERASE CHAIN REACTION FOR ANTEMORTEM DETECTION OF MYCOBACTERIA IN AMAZON PARROTS (AMAZONA AESTIVA) AND ORANGE-WINGED AMAZONS (AMAZONA AMAZONICA)
Arianne Costa Baquião,
Janaina Oliveira Luna,
Aziz Orro Medina,
Luiz Francisco Sanfilippo,
Maria Jacinta de Faria,
Manuel Armando Azevedo dos Santos
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avian tuberculosis
Mycobacterium
nested polymerase chain reaction
parrots
TUBERCULOSIS