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1 March 2016 AVIAN POXVIRUS INFECTION IN A FLAMINGO (PHOENICOPTERUS RUBER) OF THE LISBON ZOO
Ana M. Henriques, Teresa Fagulha, Margarida Duarte, Fernanda Ramos, Sílvia C. Barros, Tiago Luís, Rui Bernardino, Teresa L. Fernandes, Narciso Lapão, José Ferreira da Silva, Miguel Fevereiro
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Abstract

Avian poxviruses (APV) are very large viruses spread worldwide in a variety of hosts. They are responsible for a disease usually referred to as pox, mainly characterized by nodular lesions on feather-free regions of the body. On May 2010, a young American flamingo (Phoenicopterus ruber) of the Lisbon Zoo (Portugal) developed a nodular lesion suggestive of poxvirus infection on its right foot. Avipoxvirus was isolated from the lesion and a fragment of the P4b-encoding gene was amplified by polymerase chain reaction. The nucleotide sequence of the amplicon was determined and analyzed. A close relationship (100% identity) was observed between the flamingo poxvirus and isolates from great bustard (Hungary 2005), house sparrow (Morocco 2009), MacQueen's bustard (Morocco 2011), and Houbara bustard (Morocco 2010 and 2011), suggesting interspecies transmission as a possible source of infection. To strengthen the investigation, the 5′ and 3′ ends of genes cnpv186 and cnpv 187, respectively, were also analyzed. The cnpv186-187 fragment exhibited 100% identity with MacQueen's bustard and Houbara bustard isolates, both from Morocco 2011. Phylogenetic analyses based in both fragments grouped the flamingo isolate consistently within clade B2 of canarypox. However, the phylogenetic relationships among the different representatives of avian poxviruses were more comprehensive in the tree based on the concatenated coding sequences of the cnpv186-187 fragment, rather than on the P4b-coding gene. The clearer displacement and distribution of the isolates regarding their host species in this last tree suggests the potential usefulness of this genomic region to refine avian poxvirus classification.

Copyright 2016 by American Association of Zoo Veterinarians
Ana M. Henriques, Teresa Fagulha, Margarida Duarte, Fernanda Ramos, Sílvia C. Barros, Tiago Luís, Rui Bernardino, Teresa L. Fernandes, Narciso Lapão, José Ferreira da Silva, and Miguel Fevereiro "AVIAN POXVIRUS INFECTION IN A FLAMINGO (PHOENICOPTERUS RUBER) OF THE LISBON ZOO," Journal of Zoo and Wildlife Medicine 47(1), 161-174, (1 March 2016). https://doi.org/10.1638/2011-0101.1
Received: 17 May 2011; Published: 1 March 2016
JOURNAL ARTICLE
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KEYWORDS
avian poxvirus
Flamingo
Phoenicopterus ruber
phylogenetic analysis
poxvirus
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