An adult, captive-born eastern massasauga (Sistrurus catenatus catenatus) was examined for a subcutaneous abscess and fistula cranial to the vent. The wound improved initially with lavage and systemic antibiotic therapy, but multiple, scattered, small subcutaneous nodules later developed over the ventrum and lateral aspects of the body. Examination of fine needle aspirates from these nodules revealed granulomatous inflammation and fungal elements morphologically consistent with Ophidiomyces ophiodiicola. The animal died before antifungal therapy could be implemented. At necropsy, fungal granulomas were also present in the kidneys, liver, lung, air sac, ovary, and spleen. This case report describes an atypical presentation of systemic ophidiomycosis in a captive-born snake.