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1 June 2017 DIAGNOSIS OF PREGNANCY IN BARBARY SHEEP (AMMOTRAGUS LERVIA) USING A BOVINE ASSAY FOR PREGNANCY-ASSOCIATED GLYCOPROTEINS
Benjamin Lamglait, Thomas Rambaud
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Abstract

Pregnancy diagnosis is an important part in reproduction management of wild ruminants involved in free-ranging and captive programs. Pregnancy-associated glycoproteins (PAGs) are placenta-expressed proteins released into maternal blood circulation. Tests with high specificity have been developed and validated in domestic species and have been used in some wild ungulate species. One hundred and seventeen serum samples collected from 72 mature female Barbary sheep (Ammotragus lervia) were tested using a commercial enzyme-linked immunosorbent assay (ELISA) test. Pregnancy was determined either postmortem (n = 5) or by visualization of parturition (n = 33). The other sera were controls from known nonpregnant females (n = 71). The following values were obtained: sensitivity = 100.0%, specificity = 95.8%. Using a commercial ELISA for the detection of PAGs appears to be a rapid, inexpensive, and accurate test for pregnancy diagnosis in the endangered Barbary sheep. The number of offspring cannot be determined with this method.

Copyright 2017 by American Association of Zoo Veterinarians
Benjamin Lamglait and Thomas Rambaud "DIAGNOSIS OF PREGNANCY IN BARBARY SHEEP (AMMOTRAGUS LERVIA) USING A BOVINE ASSAY FOR PREGNANCY-ASSOCIATED GLYCOPROTEINS," Journal of Zoo and Wildlife Medicine 48(2), 525-528, (1 June 2017). https://doi.org/10.1638/2016-0129.1
Received: 9 February 2017; Published: 1 June 2017
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KEYWORDS
Ammotragus lervia
Barbary sheep
ELISA
PAG
pregnancy
pregnancy-associated glycoproteins
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