A single 8 mg/kg dose of Cefovecin (Convenia®) was administered intramuscularly in the hindlimb of eight anesthetized captive tigers (Panthera tigris) and serial blood samples were collected over the next 56 days to determine pharmacokinetic characteristics. High-performance liquid chromatography was utilized to determine plasma levels, and demonstrated a mean terminal half-life of 227.8 ± 29.3 hr. Cefovecin, >1 μg/ml, was detected in plasma levels up to 56 days postinjection. Protein binding of tiger plasma for cefovecin was 98%. The long duration of therapeutic plasma concentrations and lack of adverse effects make cefovecin a useful antimicrobial drug for tigers.