Published research on the health status of wild bald eagles (Haliaeetus leucocephalus) in Eastern Canada is limited. This study examined the health status of 53 nestling bald eagles on Prince Edward Island in the summers of 2012 and 2013, including biochemistry reference intervals, a summary of hematological parameters, and background lead accumulation. Baseline lead levels of greater than 0.02 ppm were noted in nine nestlings, and the odds of lead accumulation decreased with increased weight and body condition score. Biochemical reference intervals were developed using a method outlined by the American Society of Veterinary Clinical Pathology. These intervals can vary between populations based on age and geographic area. These findings will therefore be useful in further research and clinical cases within this population, as well as further research into subclinical lead accumulation in growing raptors.