A new pteridophyte species, Woodsia guizhouensis P. S. Wang, Q. Luo & Li Bing Zhang (Woodsiaceae), is described and illustrated from higher elevational areas in Guizhou, China. It is morphologically most similar to W. andersonii (Bedd.) Christ and W. rosthorniana Diels. Woodsia guizhouensis is only 3–8 cm tall, its rhizome scales are light brown with sparsely fimbriate margins, its pinnae are short-petiolulate, and its pinna bases are cordate or sometimes rounded. The perispore sculpture of W. guizhouensis pollen is cristate, with numerous acanthoid projections and a few irregular perforations. Woodsia guizhouensis is only known from four localities in western Guizhou and is considered to be Endangered (EN), based on IUCN Red List criteria.