The new species Gentiana woodii J. S. Pringle, from Departments Cochabamba and La Paz, Bolivia, and Gentianella glenniae J. S. Pringle, from Departments Amazonas and Cajamarca, Peru, are described. Gentiana woodii differs from G. sedifolia Kunth and G. sirensis J. S. Pringle in its conspicuously white-margined leaves, which are more widely spaced distally, and from G. microphylla Griseb. in its monocarpic habit and narrower leaves. Gentianella glenniae differs from G. ericothamna (Gilg) Zarucchi in its more elongated inflorescences, triangular rather than linear calyx lobes, and more deeply lobed corollas, and from G. oreosilene (Gilg) J. S. Pringle in its smaller flowers, more deeply lobed corollas, and narrowly lanceolate rather than obovate corolla lobes.