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1 September 2001 Isomerization of DNA-bound Distilbazolium Ligand Induced by Electron Transfer from Photoexcited Tris(1,10-phenanthroline)Ru(II)
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Abstract

The ability of the DNA duplex to behave as an efficient organized medium for cistrans isomerization induced by electron transfer (ET) has been explored. Isomerization studies, luminescence quenching and DNA photocleavage assays show that photoexcited Ru(1,10-phenanthroline [phen])32 transfers an electron to E,Z1,4-bis[2-(1-methylpyridinium-4-yl)vinyl]benzene (E,Z pMPVB), which subsequently undergoes one-way isomerization to E,E pMPVB. The unusual feature of the system is manifested by the lack of friction that is usually imposed on the photoisomerizable ligand by highly organized media. The apparent rate of ET in DNA increases when compared with the homogeneous solution. However, after correction for the local concentration of the reagents onto the biopolymer, the rate constant becomes independent of the DNA concentration and is at least 4 × 102 times smaller than that in the homogeneous aqueous solution. Using the photoinduced isomerization system, a large enhancement in the efficiency of single-strand break formation was found in plasmid DNA over that for Ru(phen)3 2 alone using irradiation at λ > 480 nm.

Bernard Juskowiak, Agnieszka Dominiak, Shigeori Takenaka, and Makoto Takagi "Isomerization of DNA-bound Distilbazolium Ligand Induced by Electron Transfer from Photoexcited Tris(1,10-phenanthroline)Ru(II)," Photochemistry and Photobiology 74(3), 391-400, (1 September 2001). https://doi.org/10.1562/0031-8655(2001)074<0391:IODBDL>2.0.CO;2
Received: 25 January 2001; Accepted: 1 June 2001; Published: 1 September 2001
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