Translator Disclaimer
1 April 2002 In Vitro Investigation on the pH Dependence of the Absorption and Fluorescence Properties of the Photosensitizer mTHPC
Author Affiliations +
Abstract

Fluorescence excitation efficiency is of great importance for photodynamic diagnosis. Because usually a difference in the interstitial pH between normal and tumor tissue occurs, it is necessary to assess the impact of pH on the fluorescence emission intensity of the photosensitizer meta-tetrahydroxyphenylchlorin (mTHPC) in this context. The results obtained by in vitro fluorescence measurements clearly indicate that pH values below 6 lead to a significant decrease in the fluorescence intensity. In the physiological range of pH 6.5–7.2, however, no pH dependence was found. Besides the decrease in the fluorescence intensity of mTHPC for pH < 6, changes in the spectral shape of the absorption were found. These changes can be utilized for “dual-wavelength ratio imaging,” using mTHPC as a pH-sensitive indicator with the excitation pair 405 nm/436 nm in the range of pH 3.5–6.

Andreas Zimmermann, Monika Ritsch-Marte, and Herwig Kostron "In Vitro Investigation on the pH Dependence of the Absorption and Fluorescence Properties of the Photosensitizer mTHPC," Photochemistry and Photobiology 75(4), 335-338, (1 April 2002). https://doi.org/10.1562/0031-8655(2002)075<0335:IVIOTP>2.0.CO;2
Received: 28 March 2001; Accepted: 1 January 2002; Published: 1 April 2002
JOURNAL ARTICLE
4 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top