Translator Disclaimer
1 March 2003 Dose and Timing of the First Light Fraction in Two-fold Illumination Schemes for Topical ALA-mediated Photodynamic Therapy of Hairless Mouse Skin
Author Affiliations +
Abstract

A fractionated illumination scheme in which a cumulative fluence of 100 J cm−2 is delivered in two equal light fractions separated by a dark interval of 2 h has been shown to considerably increase the efficacy of 5-aminolevulinic acid (ALA)–photodynamic therapy (PDT). The efficacy of such a scheme is further increased if the fluence of the first light fraction is reduced to 5 J cm−2. We have investigated the relationship between the PDT response and the kinetics of protoporphyrin IX (PpIX) fluorescence in the SKH1 HR hairless mouse for first fraction fluences below 5 J cm−2 delivered 4 h after the application of ALA and 10 J cm−2 delivered 2 h after the application of ALA. Illumination is performed using 514 nm at a fluence rate of 50 mW cm−2. Reducing the fluence of the first fraction to 2.5 J cm−2 does not result in significantly different visual skin damage. The PDT response, however, is significantly reduced if the fluence is lowered to 1 J cm−2, but this illumination scheme (1 99 J cm−2) remains significantly more effective than a single illumination of 100 J cm−2. A first light fraction of 10 J cm−2 can be delivered 2 h earlier, 2 h after the application of ALA, without significant reduction in the PDT response compared with 5 95 J cm−2 delivered 4 and 6 h after the application of ALA. The kinetics of PpIX fluorescence are consistent with those reported previously by us and do not explain the significant increase in PDT response with a two-fold illumination scheme. Histological sections of the illuminated volume showed a trend toward increasing extent and depth of necrosis for the two-fold illumination scheme in which the first light fraction is 5 J cm−2, compared with a single illumination scheme.

Dominic J. Robinson, Henriëtte S. de Bruijn, Willem M. Star, and Henricus J. C M. Sterenborg "Dose and Timing of the First Light Fraction in Two-fold Illumination Schemes for Topical ALA-mediated Photodynamic Therapy of Hairless Mouse Skin," Photochemistry and Photobiology 77(3), 319-323, (1 March 2003). https://doi.org/10.1562/0031-8655(2003)077<0319:DATOTF>2.0.CO;2
Received: 2 August 2002; Accepted: 1 December 2002; Published: 1 March 2003
JOURNAL ARTICLE
5 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top