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1 October 2003 A New Approach for the Detection of Carbon-centered Radicals in Enzymatic Processes Using Prefluorescent Probes
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Abstract

In this communication we propose a novel application for prefluorescent probes in the detection of free carbon-centered radicals in enzymatic processes. Prefluorescent probes combine a fluorescent moiety tethered to a paramagnetic nitroxide that acts as a fluorescence quencher. Trapping of a radical by the nitroxide group restores the fluorescence properties. The increase in fluorescence intensity with time reflects the formation and quenching of carbon-centered radicals and can be used for the quantitative evaluation of yields and kinetics. As a test system we used horseradish peroxidase, an oxidoreductase that is widely accepted to operate by a radical-mediated mechanism. We used the prefluorescent probe (quinoline-TEMPO), where a quinoline moiety has been tethered to 2,2,6,6-tetramethylpiperidin-1-oxyl.

Marius G. Ivan and J. C. Scaiano "A New Approach for the Detection of Carbon-centered Radicals in Enzymatic Processes Using Prefluorescent Probes," Photochemistry and Photobiology 78(4), 416-419, (1 October 2003). https://doi.org/10.1562/0031-8655(2003)078<0416:ANAFTD>2.0.CO;2
Received: 7 July 2003; Accepted: 1 August 2003; Published: 1 October 2003
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