Singlet oxygen (1O2) is the most important cytotoxic agent in photodynamic therapy (PDT). The feasibility of using a chemiluminescence (CL) probe, 3,7-dihydro-6-[4-(2-(N′-(5-fluoresceinyl)thioureido)ethoxy)phenyl]-2-methylimidazo{1,2-a}pyrazin-3-one sodium salt (fluoresceinyl Cypridina luciferin analog, FCLA), to monitor 1O2 production during PDT is evaluated in vitro. Lymphoma cells were treated with various protocols of PDT. The results show that the FCLA-CL production during PDT is linearly related to the corresponding cytotoxicity, regardless of the treatment protocol. With minimum cytotoxicity and interference to the PDT treatment outcome, the FCLA-CL system is an effective means to quantify PDT 1O2 production and may provide an alternative real-time dosimeter.
How to translate text using browser tools
1 November 2005
Feasibility of Using Fluoresceinyl Cypridina Luciferin Analog in a Novel Chemiluminescence Method for Real-time Photodynamic Therapy Dosimetry
Yanfang Qin,
Da Xing,
Shiming Luo,
Jing Zhou,
Xueyun Zhong,
Qun Chen
ACCESS THE FULL ARTICLE
It is not available for individual sale.
This article is only available to subscribers.
It is not available for individual sale.
It is not available for individual sale.
Photochemistry and Photobiology
Vol. 81 • No. 6
November 2005
Vol. 81 • No. 6
November 2005