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1 May 2006 Inhibitory Effects of Mizolastine on Ultraviolet B–Induced Leukotriene B4 Production and 5-Lipoxygenase Expression in Normal Human Dermal Fibroblasts In Vitro
Yan Yan, Baoxi Wang, Ya-gang Zuo, Tao Qu
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Abstract

Ultraviolet B (UVB) irradiation exerts hazardous effects such as acute photodamage, skin cancer and photoaging. In this study we evaluated the protective effects of a nonsedative histamine H1-receptor antagonist, mizolastine, on UVB-exposed skin dermal fibroblasts. Therefore, primary human skin fibroblasts were incubated with mizolastine or dexamethasone after 100 mJ/cm2 UVB irradiation. Leukotriene B4 (LTB4) in fibroblast supernatants was detected with enzyme immunoassays, expression of 5-lipoxygenase (5-LOX) messenger RNA (mRNA) in skin fibroblasts was examined by reverse transcriptase–polymerase chain reaction and expression of 5-LOX protein was measured by immune blotting and immunofluorescent staining with rabbit anti-human 5-LOX antibody. It was found that 0.01 mM mizolastine inhibited UVB-induced LTB4 production from skin fibroblasts at 12, 24 and 36 h. Meanwhile, mizolastine down-regulated 5-LOX mRNA expression and inhibited 5-LOX translocation from nucleus to cytoplasm in fibroblasts. On the basis of these findings, we propose that mizolastine might play a protective role in the pathogenesis of UV radiation–induced acute photodamage of the skin.

Yan Yan, Baoxi Wang, Ya-gang Zuo, and Tao Qu "Inhibitory Effects of Mizolastine on Ultraviolet B–Induced Leukotriene B4 Production and 5-Lipoxygenase Expression in Normal Human Dermal Fibroblasts In Vitro," Photochemistry and Photobiology 82(3), 665-669, (1 May 2006). https://doi.org/10.1562/2005-08-17-RA-652
Received: 17 August 2005; Accepted: 1 January 2006; Published: 1 May 2006
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